This gene product belongs to the SCAMP family of proteins which are secretory carrier membrane proteins. They function as carriers to the cell surface in post-golgi recycling pathways. Different family members are highly related products of distinct genes, and are usually expressed together. These findings suggest that the SCAMPs may function at the same site during vesicular transport rather than in separate pathways. Alternate splicing results in multiple transcript variants.

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Western blotting analysis using SCAMP2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SCAMP2 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using SCAMP2 antibody , Top panel: wild-type (WT); Bottom panal: SCAMP2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; SCAMP2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Flow cytometric analysis of SCAMP2 expression in HAP-1 cells using SCAMP2 antibody . Green, isotype control; red, SCAMP2.

Western blotting analysis using SCAMP2 antibody . SCAMP2 expression in wild-type (WT) and SCAMP2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SCAMP2 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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