This gene encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of ARID1A expression in C2C12 cells using ARID1A antibody . Green, isotype control; red, ARID1A.

Western blotting analysis using ARID1A antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ARID1A antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using ARID1A antibody . ARID1A expression in wild type (WT) and ARID1A shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ARID1A antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of Hela cells using ARID1A antibody , Top panel: wild-type (WT); Bottom panal: ARID1A shRNA knockdown (KD). Nuclei were stained blue with DAPI; ARID1A was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Validation of ARID1A knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with ARID1A antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of C2C12 cells with ARID1A antibody . Nuclei were stained blue with DAPI; ARID1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

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