This gene encodes a member of the NipSnap family of proteins that may be involved in vesicular transport. The encoded protein is localized to mitochondria and plays a role in oxidative phosphorylation. A pseudogene of this gene is located on the long arm of chromosome 2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

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Validation of NIPSNAP2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with NIPSNAP2 antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of NIPSNAP2 expression in HepG2 cells using NIPSNAP2 antibody . Green, isotype control; red, NIPSNAP2.

Western blotting analysis using NIPSNAP2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NIPSNAP2 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using NIPSNAP2 antibody . NIPSNAP2 expression in wild-type (WT) and NIPSNAP2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NIPSNAP2 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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