AKR1C1: This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reaction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Western blotting analysis using AKR1C1 / AKR1C2 antibody . AKR1C1 / AKR1C2 expression in wild type (WT) and AKR1C1 shRNA knockdown (KD) HeLa cells with 30 μg of Total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with AKR1C1 / AKR1C2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Flow cytometric analysis of AKR1C1/AKR1C2 expression in HeLa cells using AKR1C1/AKR1C2 antibody . Green, isotype control; red, AKR1C1/AKR1C2.

Immunocytochemical staining of Hela cells with AKR1C1/AKR1C2 antibody . Nuclei were stained blue with DAPI; AKR1C1/AKR1C2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using AKR1C1/AKR1C2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AKR1C1/AKR1C2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using AKR1C1 / AKR1C2 antibody , Top panel: wild-type (WT); Bottom panal: AKR1C1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; AKR1C1 / AKR1C2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

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