The encoded protein catalyzes the last step of the mitochondrial fatty acid beta-oxidation spiral. Unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal.

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Flow cytometric analysis of Acetyl-CoA acyltransferase 2 expression in C2C12 cells using Acetyl-CoA acyltransferase 2 antibody . Green, isotype control; red, Acetyl-CoA acyltransferase 2.

Immunocytochemical staining of C2C12 cells with Acetyl-CoA acyltransferase 2 antibody . Nuclei were stained blue with DAPI; Acetyl-CoA acyltransferase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using Acetyl-CoA acyltransferase 2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Acetyl-CoA acyltransferase 2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Acetyl-CoA acyltransferase 2 antibody . Acetyl-CoA acyltransferase 2 expression in wild type (WT) and Acetyl-CoA acyltransferase 2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Acetyl-CoA acyltransferase 2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using Acetyl-CoA acyltransferase 2 antibody , Top panel: wild-type (WT); Bottom panal: Acetyl-CoA acyltransferase 2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acetyl-CoA acyltransferase 2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Validation of Acetyl-CoA acyltransferase 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Acetyl-CoA acyltransferase 2 antibody and analyzed using BD flow cytometer.

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