This gene encodes a component of the heteromeric ESCRT-III complex (Endosomal Sorting Complex Required for Transport III) that functions in the recycling or degradation of cell surface receptors. ESCRT-III functions in the concentration and invagination of ubiquitinated endosomal cargos into intralumenal vesicles. The protein encoded by this gene is found as a monomer in the cytosol or as an oligomer in ESCRT-III complexes on endosomal membranes. It is expressed in neurons of all major regions of the brain. Mutations in this gene result in one form of familial frontotemporal lobar degeneration.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Validation of CHMP2B knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with CHMP2B antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using CHMP2B antibody , Top panel: wild-type (WT); Bottom panal: CHMP2B shRNA knockdown (KD). Nuclei were stained blue with DAPI; CHMP2B was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Flow cytometric analysis of CHMP2B expression in HepG2 cells using CHMP2B antibody . Green, isotype control; red, CHMP2B.

Immunocytochemical staining of HepG2 cells with CHMP2B antibody . Nuclei were stained blue with DAPI; CHMP2B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using CHMP2B antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CHMP2B antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit . CHMP2B, charged multivesicular body protein 2B.

Western blotting analysis using CHMP2B antibody . CHMP2B expression in wild type (WT) and CHMP2B shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with CHMP2B antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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