The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Validation of Chromodomain helicase DNA binding protein 4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Chromodomain helicase DNA binding protein 4 antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of Chromodomain helicase DNA binding protein 4 expression in HepG2 cells using Chromodomain helicase DNA binding protein 4 antibody . Green, isotype control; red, Chromodomain helicase DNA binding protein 4.

Immunocytochemical staining of HepG2 cells with Chromodomain helicase DNA binding protein 4 antibody . Nuclei were stained blue with DAPI; Chromodomain helicase DNA binding protein 4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using Chromodomain helicase DNA binding protein 4 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Chromodomain helicase DNA binding protein 4 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Chromodomain helicase DNA binding protein 4 antibody . Chromodomain helicase DNA binding protein 4 expression in wild type (WT) and Chromodomain helicase DNA binding protein 4 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Chromodomain helicase DNA binding protein 4 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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