This gene encodes a protein that is similar to some members of the ERM (ezrin, radixin, moesin) family of proteins that link cytoskeletal components with proteins in the cell membrane. The encoded protein is involved in regulation of contact-dependent inhibition of cell proliferation and functions in cell-cell adhesion and transmembrane signaling. The encoded protein has been shown to interact with cell-surface proteins, proteins involved in cytoskeletal dynamics, and proteins involved in regulating ion transport. Disruption of this protein's function has been implicated in tumorigenesis and metastasis. Mutations in this gene are associated with neurofibromatosis type II which is characterized by nervous system and skin tumors and ocular abnormalities.

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Western blotting analysis using NF2 / Merlin antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NF2 / Merlin antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Flow cytometric analysis of NF2 / Merlin expression in HAP-1 cells using NF2 / Merlin antibody . Green, isotype control; red, NF2 / Merlin.

Immunocytochemical staining of HAP-1 cells with NF2 / Merlin antibody . Nuclei were stained blue with DAPI; NF2 / Merlin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using NF2 / Merlin antibody . NF2 / Merlin expression in wild type (WT) and NF2 / Merlin shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NF2 / Merline antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Validation of NF2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with NF2 / Merlin antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using NF2 / Merlin antibody , Top panel: wild-type (WT); Bottom panal: NF2 / Merlin shRNA knockdown (KD). Nuclei were stained blue with DAPI; NF2 / Merlin was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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