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Flow cytometric analysis of CHD1L expression in HT-1080 cells using CHD1L antibody . Green, isotype control; red, CHD1L.
Immunocytochemical staining of HT-1080 cells with CHD1L antibody . Nuclei were stained blue with DAPI; CHD1L was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using CHD1L antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CHD1L antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .
Western blotting analysis using CHD1L antibody . CHD1L expression in wild-type (WT) and CHD1L knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CHD1L antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .
