The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000

本产品仅用作科学研究！

Flow cytometric analysis of Protein kinase N1 expression in C2C12 cells using Protein kinase N1 antibody . Green, isotype control; red, Protein kinase N1.

Western blotting analysis using protein kinase N1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with protein kinase N1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using protein kinase N1 antibody . Protein kinase N1A expression in wild-type (WT) and protein kinase N1 (PKN1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with protein kinase N1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of Protein kinase N1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Protein kinase N1 antibody and analyzed using BD flow cytometer.

Store at -20℃ for one year.