This gene encodes an F-box-containing protein that is a component of an SCF-type E3 ubiquitin ligase complex that regulates the onset of cell division. The G2/M transition in the cell cycle requires the interaction of the proteins cyclin B1 and cyclin-dependent kinase 1. The activated ubiquitin ligase complex targets the protein cyclin B1 for degradation, preventing this transition to mitosis.

推荐稀释比 WB: 1/200-1/1000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of ZC3HC1 expression in HeLa cells using ZC3HC1 antibody . Green, isotype control; red, ZC3HC1.

Immunocytochemical staining of Hela cells with ZC3HC1 protein antibody . Nuclei were stained blue with DAPI; ZC3HC1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using ZC3HC1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ZC3HC1 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using ZC3HC1 antibody . ZC3HC1 expression in wild-type (WT) and ZC3HC1 knockdown (KD) HSHC cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ZC3HC1 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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