The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase mediates the signaling transduction induced by TGF beta and morphogenetic protein (BMP), and controls a variety of cell functions including transcription regulation and apoptosis. In response to IL-1, this protein forms a kinase complex including TRAF6, MAP3K7P1/TAB1 and MAP3K7P2/TAB2; this complex is required for the activation of nuclear factor kappa B. This kinase can also activate MAPK8/JNK, MAP2K4/MKK4, and thus plays a role in the cell response to environmental stresses. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Western blotting analysis using MAP3K7 antibody . MAP3K7 expression in wild type (WT) and MAP3K7 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with MAP3K7 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using MAP3K7 antibody , Top panel: wild-type (WT); Bottom panal: MAP3K7 shRNA knockdown (KD). Nuclei were stained blue with DAPI;MAP3K7 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Flow cytometric analysis of MAP3K7 expression in HeLa cells using MAP3K7 antibody . Green, isotype control; red, MAP3K7.

Immunocytochemical staining of Hela cells with MAP3K7 antibody . Nuclei were stained blue with DAPI; MAP3K7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Scale bar, 20 μm.

Western blotting analysis using MAP3K7 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAP3K7 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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