Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms.

推荐稀释比 WB: 1/2000-1/10000; FC: 1/200-1/2000

本产品仅用作科学研究！

Flow cytometric analysis of PI3 Kinase p85 alpha expression in C2C12 cells using PI3 Kinase p85 alpha antibody . Green, isotype control; red, PI3 Kinase p85 alpha.

Western blotting analysis using PI3 Kinase p85 alpha antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PI3 Kinase p85 alpha antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using PI3 Kinase p85 alpha antibody . PI3 Kinase p85 alpha expression in wild-type (WT) andPIK3R1 knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PI3 Kinase p85 alpha antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Store at -20℃ for one year.