Enables protein serine/threonine kinase activity. Involved in spermatogenesis. Predicted to be located in manchette. Predicted to be active in nucleus. Implicated in colorectal cancer; depressive disorder; and spermatogenic failure 93.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000

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Western blotting analysis using serine/threonine kinase 33 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with serine/threonine kinase 33 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using serine/threonine kinase 33 antibody . Serine/threonine kinase 33 expression in wild-type (WT) and serine/threonine kinase 33 (STK33) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with serine/threonine kinase 33 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Flow cytometric analysis of Serine/threonine kinase 33 expression in HT-1080 cells using Serine/threonine kinase 33 antibody . Green, isotype control; red, Serine/threonine kinase 33.

Validation of Serine/threonine kinase 33 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Serine/threonine kinase 33 antibody and analyzed using BD flow cytometer.

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