The protein encoded by this gene catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

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Flow cytometric analysis of Glycogen synthase 1 expression in HepG2 cells using Glycogen synthase 1 antibody . Green, isotype control; red, Glycogen synthase 1.

Western blotting analysis using glycogen synthase 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with glycogen synthase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using glycogen synthase 1 antibody . Glycogen synthase 1 expression in wild-type (WT) and glycogen synthase 1 (GYS1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with glycogen synthase 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of Glycogen synthase 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Glycogen synthase 1 antibody and analyzed using BD flow cytometer.

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