This gene encodes a syntaxin-binding protein. The encoded protein appears to play a role in release of neurotransmitters via regulation of syntaxin, a transmembrane attachment protein receptor. Mutations in this gene have been associated with infantile epileptic encephalopathy-4. Alternatively spliced transcript variants have been described.

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Western blotting analysis using Syntaxin binding protein 1 antibody . Syntaxin binding protein 1 expression in wild type (WT) and syntaxin binding protein 1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Syntaxin binding protein 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Flow cytometric analysis of Syntaxin binding protein 1 expression in C2C12 cells using Syntaxin binding protein 1 antibody . Green, isotype control; red, Syntaxin binding protein 1.

Immunocytochemical staining of C2C12 cells with Syntaxin binding protein 1 antibody . Nuclei were stained blue with DAPI; Syntaxin binding protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Syntaxin binding protein 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Syntaxin binding protein 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of Syntaxin binding protein 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Syntaxin binding protein 1 antibody and analyzed using BD flow cytometer.

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