The protein encoded by this gene interacts with poly(A)-binding protein and with the cap-binding complex eIF4A. It is involved in translational initiation and protein biosynthesis. Overexpression of this gene in COS7 cells stimulates translation. Alternative splicing occurs at this locus and three transcript variants encoding three distinct isoforms have been identified.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/100-1/1000

本产品仅用作科学研究！

Flow cytometric analysis of PAIP1 expression in HepG2 cells using PAIP1 antibody . Green, isotype control; red, PAIP1.

Western blotting analysis using PAIP1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PAIP1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using PAIP1 antibody . PAIP1 expression in wild-type (WT) and PAIP1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PAIP1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of PAIP1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with PAIP1 antibody and analyzed using BD flow cytometer.

Store at -20℃ for one year.