This gene encodes a type 1 transmembrane protein that mediates Ca2+ influx after depletion of intracellular Ca2+ stores by gating of store-operated Ca2+ influx channels (SOCs). It is one of several genes located in the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocrotical carcinoma, and lung, ovarian, and breast cancer. This gene may play a role in malignancies and disease that involve this region, as well as early hematopoiesis, by mediating attachment to stromal cells. Mutations in this gene are associated with fatal classic Kaposi sarcoma, immunodeficiency due to defects in store-operated calcium entry (SOCE) in fibroblasts, ectodermal dysplasia and tubular aggregate myopathy. This gene is oriented in a head-to-tail configuration with the ribonucleotide reductase 1 gene (RRM1), with the 3' end of this gene situated 1.6 kb from the 5' end of the RRM1 gene. Alternative splicing of this gene results in multiple transcript variants.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Western blotting analysis using stromal interaction molecule 1 antibody . Stromal interaction molecule 1 expression in wild type (WT) and stromal interaction molecule 1 (STIM1) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with stromal interaction molecule 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Flow cytometric analysis of Stromal interaction molecule 1 expression in HeLa cells using Stromal interaction molecule 1 antibody . Green, isotype control; red, Stromal interaction molecule 1.

Immunocytochemical staining of HeLa cells with Stromal interaction molecule 1 antibody . Nuclei were stained blue with DAPI; Stromal interaction molecule 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Stromal interaction molecule 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Stromal interaction molecule 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using Stromal interaction molecule 1 antibody , Top panel: wild-type (WT); Bottom panal: Stromal interaction molecule 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Stromal interaction molecule 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Validation of Stromal interaction molecule 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Stromal interaction molecule 1 antibody and analyzed using BD flow cytometer.

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