Retinoid X receptors (RXRs) and retinoic acid receptors (RARs) are nuclear receptors that mediate the biological effects of retinoids by their involvement in retinoic acid-mediated gene activation. These receptors function as transcription factors by binding as homodimers or heterodimers to specific sequences in the promoters of target genes. The protein encoded by this gene is a member of the steroid and thyroid hormone receptor superfamily of transcriptional regulators. Alternative splicing of this gene results in multiple transcript variants.

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Flow cytometric analysis of Retinoid X receptor alpha expression in HeLa cells using Retinoid X receptor alpha antibody . Green, isotype control; red, Retinoid X receptor alpha.

Immunocytochemical staining of Hela cells with Retinoid X receptor alpha antibody . Nuclei were stained blue with DAPI; Retinoid X receptor alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Retinoid X receptor alpha antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Retinoid X receptor alpha antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Retinoid X receptor alpha antibody . Retinoid X receptor alpha expression in wild type (WT) and retinoid X receptor alpha shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Retinoid X receptor alpha antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of Retinoid X receptor alpha knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Retinoid X receptor alpha antibody and analyzed using BD flow cytometer.

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