The protein encoded by this gene is highly conserved and is involved in cell survival after damage or stress, increase in DNA polymerase epsilon activity, maintenance of telomere length, and epithelial cell morphogenesis. The encoded protein also plays a role in the circadian rhythm autoregulatory loop, interacting with the PERIOD genes (PER1, PER2, and PER3) and others to downregulate activation of PER1 by CLOCK/ARNTL. Changes in this gene or its expression may promote prostate cancer, lung cancer, breast cancer, and mental disorders.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Validation of Timeless circadian regulator knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Timeless circadian regulator antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of Timeless circadian regulator expression in HepG2 cells using Timeless circadian regulator antibody . Green, isotype control; red, Timeless circadian regulator.

Immunocytochemical staining of HepG2 cells with Timeless circadian regulator antibody . Nuclei were stained blue with DAPI; Timeless circadian regulator was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Timeless circadian regulator antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Timeless circadian regulator antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using timeless circadian regulator antibody . Timeless circadian regulator expression in wild-type (WT) and timeless circadian regulator (TIMELESS) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with timeless circadian regulator antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using Timeless circadian regulator antibody , Top panel: wild-type (WT); Bottom panal: Timeless circadian regulator shRNA knockdown (KD). Nuclei were stained blue with DAPI; Timeless circadian regulator was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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