This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

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Flow cytometric analysis of Phospho-RSK1(S380) expression in C2C12 cells using Phospho-RSK1(S380) antibody . Green, isotype control; red, Phospho-RSK1(S380).

Immunocytochemical staining of HeLa cells with Phospho-RSK1(S380) antibody . Nuclei were stained blue with DAPI; Phospho-RSK1(S380) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Phospho-RSK1(S380) antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospho-RSK1(S380) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Phospho-RSK1(S380) antibody . Phospho-RSK1(S380) expression in wild type (WT) and phospho-RSK1(S380) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Phospho-RSK1(S380) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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