This gene encodes an integral membrane protein that belongs to the synaptobrevin/vesicle-associated membrane protein subfamily of soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). The encoded protein is involved in the fusion of synaptic vesicles with the presynaptic membrane.

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Flow cytometric analysis of Vesicle associated membrane protein 8 expression in HepG2 cells using Vesicle associated membrane protein 8 antibody . Green, isotype control; red, Vesicle associated membrane protein 8.

Western blotting analysis using vesicle associated membrane protein 8 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with vesicle associated membrane protein 8 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using vesicle associated membrane protein 8 antibody . Vesicle associated membrane protein 8 expression in wild-type (WT) and vesicle associated membrane protein 8 (VAMP8) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with vesicle associated membrane protein 8 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Validation of Vesicle associated membrane protein 8 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Vesicle associated membrane protein 8 antibody and analyzed using BD flow cytometer.

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