The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene.

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Western blotting analysis using UBE2L3 antibody . UBE2L3 expression in wild-type (WT) and UBE2L3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with UBE2L3 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Validation of UBE2L3 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with UBE2L3 antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of UBE2L3 expression in HepG2 cells using UBE2L3 antibody . Green, isotype control; red, UBE2L3.

Immunocytochemical staining of HepG2 cells with UBE2L3 antibody . Nuclei were stained blue with DAPI; UBE2L3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using UBE2L3 antibody . Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with UBE2L3 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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