Predicted to enable DNA binding activity; translation elongation factor activity; and zinc ion binding activity. Predicted to be involved in positive regulation of transcription by RNA polymerase II. Predicted to act upstream of or within erythrocyte differentiation and positive regulation of DNA-templated transcription. Located in nucleolus and nucleoplasm. Part of transcription factor TFIID complex.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of Transcription elongation factor A1 expression in HepG2 cells using Transcription elongation factor A1 antibody . Green, isotype control; red, Transcription elongation factor A1.

Immunocytochemical staining of HepG2 cells with Transcription elongation factor A1 antibody . Nuclei were stained blue with DAPI; Transcription elongation factor A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using Transcription elongation factor A1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Transcription elongation factor A1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using transcription elongation factor A1 antibody . Transcription elongation factor A1 expression in wild type (WT) and transcription elongation factor A1 (TCEA1) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with transcription elongation factor A1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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