This gene encodes a member of the G protein-coupled receptor kinase family of proteins. The encoded protein phosphorylates the beta-adrenergic receptor as well as a wide range of other substrates including non-GPCR cell surface receptors, and cytoskeletal, mitochondrial, and transcription factor proteins. Data from rodent models supports a role for this gene in embryonic development, heart function and metabolism. Elevated expression of this gene has been observed in human patients with heart failure and Alzheimer's disease.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000; IHC-P: 1/100-1/200

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Flow cytometric analysis of GRK2 expression in HepG2 cells using GRK2 antibody . Green, isotype control; red, GRK2.

Immunocytochemical staining of HepG2 cells with GRK2 antibody . Nuclei were stained blue with DAPI; GRK2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using GRK2 antibody . Total cell lysates (30 µg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GRK2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using GRK2 antibody . GRK2 expression in wild-type (WT) and GRK2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with GRK2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Validation of GRK2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with GRK2 antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using GRK2 antibody , Top panel: wild-type (WT); Bottom panal: GRK2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; GRK2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Immunohistochemistry was performed on paraffin-embedded human lymphoma using GRK2 antibody . Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.

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