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Immunocytochemical staining of Hela cells using AMPK alpha 1 antibody , Top panel: wild-type (WT); Bottom panal: AMPK alpha 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; AMPK alpha 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Validation of AMPK alpha 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with AMPK alpha 1 antibody and analyzed using BD flow cytometer.
Flow cytometric analysis of AMPK alpha 1 expression in HepG2 cells using AMPK alpha 1 antibody . Green, isotype control; red, AMPK alpha 1.
Immunocytochemical staining of HepG2 cells with AMPK alpha 1 antibody . Nuclei were stained blue with DAPI; AMPK alpha 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using AMPK alpha 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AMPK alpha 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .
Western blotting analysis using AMPK alpha 1 antibody . AMPK alpha 1 expression in wild type (WT) and AMPK alpha 1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AMPK alpha 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .
