The protein encoded by this gene is a major apoprotein of the chylomicron. It binds to a specific liver and peripheral cell receptor, and is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. This gene maps to chromosome 19 in a cluster with the related apolipoprotein C1 and C2 genes. Mutations in this gene result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants.

推荐稀释比 WB: 1/1000-1/5000; ICC/IF: 1/100-1/1000

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Western blotting analysis using apolipoprotein E antibody . Apolipoprotein E expression in wild type (WT) and apolipoprotein E (APOE) knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with apolipoprotein E antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using apolipoprotein E antibody . Apolipoprotein E expression in wild type (WT) and apolipoprotein E (APOE) knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with apolipoprotein E antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using apolipoprotein E antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with apolipoprotein E antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of HAP-1 cells with Apolipoprotein E antibody . Nuclei were stained blue with DAPI; Apolipoprotein E was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

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