Malate dehydrogenase catalyzes the reversible oxidation of malate to oxaloacetate, utilizing the NAD/NADH cofactor system in the citric acid cycle. The protein encoded by this gene is localized to the mitochondria and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria. Several transcript variants encoding different isoforms have been found for this gene.

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Flow cytometric analysis of Malate dehydrogenase 2 expression in C2C12 cells using Malate dehydrogenase 2 antibody . Green, isotype control; red, Malate dehydrogenase 2.

Immunocytochemical staining of C2C12 cells with Malate dehydrogenase 2 antibody . Nuclei were stained blue with DAPI; Malate dehydrogenase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using Malate dehydrogenase 2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Malate dehydrogenase 2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Malate dehydrogenase 2 antibody . Malate dehydrogenase 2 expression in wild type (WT) and malate dehydrogenase 2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Malate dehydrogenase 2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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