The protein encoded by this gene is a calcium-dependent glycoprotein that is a member of the desmocollin subfamily of the cadherin superfamily. These desmosomal family members, along with the desmogleins, are found primarily in epithelial cells where they constitute the adhesive proteins of the desmosome cell-cell junction and are required for cell adhesion and desmosome formation. The desmosomal family members are arranged in two clusters on chromosome 18, occupying less than 650 kb combined. Mutations in this gene are a cause of hypotrichosis and recurrent skin vesicles disorder. The protein can act as an autoantigen in pemphigus diseases, and it is also considered to be a biomarker for some cancers. Alternative splicing of this gene results in multiple transcript variants.

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Flow cytometric analysis of Desmocollin 3 expression in HeLa cells using Desmocollin 3 antibody . Green, isotype control; red, Desmocollin 3.

Immunocytochemical staining of Hela cells with Desmocollin 3 antibody . Nuclei were stained blue with DAPI; Desmocollin 3 with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using desmocollin 3 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with desmocollin 3 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using desmocollin 3 antibody . Desmocollin 3 expression in wild-type (WT) and desmocollin 3 (DSC3) knockout (KO) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with desmocollin 3 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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