Predicted to enable ubiquitin binding activity. Involved in cellular response to lipopolysaccharide; macroautophagy; and positive regulation of phospholipase A2 activity. Located in cytoplasm; extracellular exosome; and nucleus.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Western blotting analysis using Phospholipase A2 activating protein antibody . Phospholipase A2 activating protein expression in wild type (WT) and phospholipase A2 activating protein shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Phospholipase A2 activating protein antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using Phospholipase A2 activating protein antibody , Top panel: wild-type (WT); Bottom panal: Phospholipase A2 activating protein shRNA knockdown (KD). Nuclei were stained blue with DAPI; Phospholipase A2 activating protein was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Validation of Phospholipase A2 activating protein knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Phospholipase A2 activating protein antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of Phospholipase A2 activating protein expression in HepG2 cells using Phospholipase A2 activating protein antibody . Green, isotype control; red, Phospholipase A2 activating protein.

Immunocytochemical staining of HepG2 cells with Phospholipase A2 activating protein antibody . Nuclei were stained blue with DAPI; Phospholipase A2 activating protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Phospholipase A2 activating protein antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Phospholipase A2 activating protein antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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