This gene encodes a member of the steroid receptor superfamily. The encoded protein mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. This gene uses two distinct promotors and translation start sites in the first exon to produce several transcript variants, both protein coding and non-protein coding. Two of the isoforms (A and B) are identical except for an additional 165 amino acids found in the N-terminus of isoform B and mediate their own response genes and physiologic effects with little overlap.

推荐稀释比 WB: 1/1000-1/5000; ICC/IF: 1/100-1/1000

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Western blotting analysis using progesterone receptor antibody . Progesterone receptor expression in wild type (WT) and progesterone receptor (PGR) knockout (KO) HSHC cells with 50 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with progesterone receptor antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of H9C2 cells with Progesterone receptor antibody . Nuclei were stained blue with DAPI; Progesterone receptor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using progesterone receptor antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with progesterone receptor antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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