Predicted to enable histone deacetylase binding activity. Involved in heart development. Predicted to be active in cytoplasm and nucleus.

推荐稀释比 WB: 1/400-1/2000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of SMYD4 expression in HepG2 cells using SMYD4 antibody . Green, isotype control; red, SMYD4.

Western blotting analysis using SMYD4 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SMYD4 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using SMYD4 antibody . SMYD4 expression in wild-type (WT) and SMYD4 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SMYD4 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of HepG2 cells with SMYD4 antibody . Nuclei were stained blue with DAPI; SMYD4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

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