This gene encodes a serine/threonine phosphatase which is a member of the protein phosphatase catalytic subunit family. Proteins in this family participate in pathways regulated by reversible phosphorylation at serine and threonine residues; many of these pathways are involved in the regulation of cell growth and differentiation. The product of this gene has been shown to participate in signaling pathways in response to hormones or cellular stress, and elevated levels of this protein may be associated with breast cancer development. Alternative splicing results in multiple transcript variants.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of Protein phosphatase 5 catalytic subunit expression in HepG2 cells using Protein phosphatase 5 catalytic subunit antibody . Green, isotype control; red, Protein phosphatase 5 catalytic subunit.

Immunocytochemical staining of HepG2 cells with Protein phosphatase 5 catalytic subunit antibody . Nuclei were stained blue with DAPI; Protein phosphatase 5 catalytic subunit was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using protein phosphatase 5 catalytic subunit antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with protein phosphatase 5 catalytic subunit antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using protein phosphatase 5 catalytic subunit antibody . Protein phosphatase 5 catalytic subunit expression in wild type (WT) and protein phosphatase 5 catalytic subunit (PPP5C) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with protein phosphatase 5 catalytic subunit antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Validation of Protein phosphatase 5 catalytic subunit knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Protein phosphatase 5 catalytic subunit antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using Protein phosphatase 5 catalytic subunit antibody , Top panel: wild-type (WT); Bottom panal: Protein phosphatase 5 catalytic subunit shRNA knockdown (KD). Nuclei were stained blue with DAPI; Protein phosphatase 5 catalytic subunit was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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