Enables ATP binding activity and protein kinase activity. Involved in several processes, including interleukin-1-mediated signaling pathway; protein modification process; and regulation of DNA-templated transcription. Located in cytoplasm and nucleoplasm.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of Phospho-MSK1 (S376) expression in H9c2 cells using Phospho-MSK1 (S376) antibody . Green, isotype control; red, Phospho-MSK1 (S376).

Immunocytochemical staining of H9c2 cells with Phospho-MSK1 (S376) antibody . Nuclei were stained blue with DAPI; Phospho-MSK1 (S376) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using phospho-MSK1 (S376) antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-MSK1 (S376) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using phospho-MSK1 (S376) antibody . Phospho-MSK1 (S376) expression in wild-type (WT) and RPS6KA5 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-MSK1 (S376) antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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