This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Western blotting analysis using Peptidase D antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Peptidase D antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Peptidase D antibody . Peptidase D expression in wild type (WT) and peptidase D shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Peptidase D antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of HepG2 cells with Peptidase D antibody . Nuclei were stained blue with DAPI; Peptidase D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Immunocytochemical staining of HeLa cells using Peptidase D antibody , Top panel: wild-type (WT); Bottom panal: Peptidase D shRNA knockdown (KD). Nuclei were stained blue with DAPI; Peptidase D was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Validation of Peptidase D knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Peptidase D antibody and analyzed using CytoFLEX.

Flow cytometric analysis of Peptidase D expression in HT-1080 cells using Peptidase D antibody . Green, isotype control; red, Peptidase D.

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