This gene encodes a protein which specifically interacts with translin, a DNA-binding protein that binds consensus sequences at breakpoint junctions of chromosomal translocations. The encoded protein contains bipartite nuclear targeting sequences that may provide nuclear transport for translin, which lacks any nuclear targeting motifs.

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Flow cytometric analysis of TSNAX expression in HeLa cells using TSNAX antibody . Green, isotype control; red, TSNAX.

Western blotting analysis using TSNAX antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TSNAX antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using TSNAX antibody . TSNAX expression in wild-type (WT) and TSNAX shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with TSNAX antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of TSNAX knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with TSNAX antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using TSNAX antibody , Top panel: wild-type (WT); Bottom panal: TSNAX shRNA knockdown (KD). Nuclei were stained blue with DAPI; TSNAX was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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