This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants.

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Validation of TNF receptor associated protein 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with TNF receptor associated protein 1 antibody and analyzed using BD flow cytometer.

Flow cytometric analysis of TNF receptor associated protein 1 expression in HepG2 cells using TNF receptor associated protein 1 antibody . Green, isotype control; red, TNF receptor associated protein 1.

Immunocytochemical staining of HepG2 cells with TNF receptor associated protein 1 antibody . Nuclei were stained blue with DAPI; TNF receptor associated protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using TNF receptor associated protein 1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with TNF receptor associated protein 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using TNF receptor associated protein 1 antibody . TNF receptor associated protein 1 expression in wild type (WT) and TNF receptor associated protein 1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with TNF receptor associated protein 1 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using TNF receptor associated protein 1 antibody , Top panel: wild-type (WT); Bottom panal: TNF receptor associated protein 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; TNF receptor associated protein 1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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