This gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of Argininosuccinate lyase expression in HT-1080 cells using Argininosuccinate lyase antibody . Green, isotype control; red, Argininosuccinate lyase.

Western blotting analysis using argininosuccinate lyase antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with argininosuccinate lyase antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using argininosuccinate lyase antibody . Argininosuccinate lyase expression in wild type (WT) and argininosuccinate lyase (ASL) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with argininosuccinate lyase antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using Argininosuccinate lyase antibody , Top panel: wild-type (WT); Bottom panal: Argininosuccinate lyase shRNA knockdown (KD). Nuclei were stained blue with DAPI;Argininosuccinate lyase was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Validation of Argininosuccinate lyase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Argininosuccinate lyase antibody and analyzed using BD flow cytometer.

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