Enables identical protein binding activity. Involved in cranial skeletal system development; establishment of protein localization; and midbody abscission. Acts upstream of or within mitotic cytokinesis. Located in Flemming body and centrosome. Implicated in multinucleated neurons, anhydramnios, renal dysplasia, cerebellar hypoplasia and hydranencephaly.

推荐稀释比 WB: 1/500-1/2500; FC: 1/100-1/1000; ICC/IF: 1/100-1/1000

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Western blotting analysis using centrosomal protein 55 antibody . Centrosomal protein 55 expression in wild-type (WT) and centrosomal protein 55 (CEP55) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with centrosomal protein 55 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Flow cytometric analysis of Centrosomal protein 55 expression in HeLa cells using Centrosomal protein 55 antibody . Green, isotype control; red, Centrosomal protein 55.

Western blotting analysis using centrosomal protein 55 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with centrosomal protein 55 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using Centrosomal protein 55 antibody , Top panel: wild-type (WT); Bottom panal: Centrosomal protein 55 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Centrosomal protein 55 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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