Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion.

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Western blotting analysis using matrix metallopeptidase 14 antibody . Matrix metallopeptidase 14 expression in wild type (WT) and matrix metallopeptidase 14 (MMP14) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with matrix metallopeptidase 14 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Flow cytometric analysis of Matrix metallopeptidase 14 expression in H9c2 cells using Matrix metallopeptidase 14 antibody . Green, isotype control; red, Matrix metallopeptidase 14.

Immunocytochemical staining of H9C2 cells with Matrix metallopeptidase 14 antibody . Nuclei were stained blue with DAPI; Matrix metallopeptidase 14 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Matrix metallopeptidase 14 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Matrix metallopeptidase 14 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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