The protein encoded by this gene is a member of the methionyl aminopeptidase family. The encoded protein functions both by protecting the alpha subunit of eukaryotic initiation factor 2 from inhibitory phosphorylation and by removing the amino-terminal methionine residue from nascent proteins. Increased expression of this gene is associated with various forms of cancer, and the anti-cancer drugs fumagillin and ovalicin inhibit the protein by irreversibly binding to its active site. Inhibitors of this gene have also been shown to be effective for the treatment of obesity. A pseudogene of this gene is located on chromosome 2. Several transcript variants encoding different isoforms have been found for this gene.

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Western blotting analysis using Methionyl aminopeptidase 2 antibody . Methionyl aminopeptidase 2 expression in wild type (WT) and methionyl aminopeptidase 2 (METAP2) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Methionyl aminopeptidase 2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Immunocytochemical staining of HeLa cells using Methionyl aminopeptidase 2 antibody , Top panel: wild-type (WT); Bottom panal: Methionyl aminopeptidase 2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Methionyl aminopeptidase 2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Validation of Methionyl aminopeptidase 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Methionyl aminopeptidase 2 antibody and analyzed using CytoFLEX.

Flow cytometric analysis of Methionyl aminopeptidase 2 expression in HepG2 cells using Methionyl aminopeptidase 2 antibody . Green, isotype control; red, Methionyl aminopeptidase 2.

Immunocytochemical staining of HepG2 cells with Methionyl aminopeptidase 2 antibody . Nuclei were stained blue with DAPI; Methionyl aminopeptidase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using Methionyl aminopeptidase 2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Methionyl aminopeptidase 2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

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