The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.

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Flow cytometric analysis of Tripartite motif containing 28 expression in HT-1080 cells using Tripartite motif containing 28 antibody . Green, isotype control; red, Tripartite motif containing 28.

Western blotting analysis using tripartite motif containing 28 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with tripartite motif containing 28 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using tripartite motif containing 28 antibody . Tripartite motif containing 28 expression in wild type (WT) and tripartite motif containing 28 (TRIM28) knockout (KO) HSHC cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with tripartite motif containing 28 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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