Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.

推荐稀释比 WB: 1/500-1/2500; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000; IHC-P: 1/100-1/200

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Flow cytometric analysis of ARHGEF1 expression in HepG2 cells using ARHGEF1 antibody . Green, isotype control; red, ARHGEF1.

Immunocytochemical staining of HepG2 cells with ARHGEF1 antibody . Nuclei were stained blue with DAPI; ARHGEF1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using ARHGEF1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ARHGEF1 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Western blotting analysis using ARHGEF1 antibody . ARHGEF1 expression in wild-type (WT) and ARHGEF1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ARHGEF1 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of ARHGEF1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with ARHGEF1 antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using ARHGEF1 antibody , Top panel: wild-type (WT); Bottom panal: ARHGEF1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acyl-CoA dehydrogenase short chain was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

Immunohistochemistry was performed on paraffin-embedded human lymphoma using ARHGEF1 antibody . Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.

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