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Immunocytochemical staining of HT-1080 cells with APMAP antibody . Nuclei were stained blue with DAPI; APMAP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Flow cytometric analysis of APMAP expression in HT-1080 cells using APMAP antibody . Green, isotype control; red, APMAP.
Western blotting analysis using APMAP antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with APMAP antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .
Western blotting analysis using APMAP antibody . APMAP expression in wild type (WT) and APMAP shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with APMAP antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .
