Somatostatin and its related peptide cortistatin exert multiple biological actions on normal and tumoral tissue targets by interacting with somatostatin receptors (SSTRs). The protein encoded by this gene is one of the SSTRs, which is a multi-pass membrane protein and belongs to the G-protein coupled receptor 1 family. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase, and different regions of this receptor molecule are required for the activation of different signaling pathways. A mutation in this gene results in somatostatin analog resistance. Alternatively spliced transcript variants have been identified in this gene.

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Flow cytometric analysis of Somatostatin receptor 5 expression in HepG2 cells using Somatostatin receptor 5 antibody . Green, isotype control; red, Somatostatin receptor 5.

Immunocytochemical staining of HepG2 cells with Somatostatin receptor 5 antibody . Nuclei were stained blue with DAPI; Somatostatin receptor 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using somatostatin receptor 5 antibody . Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with somatostatin receptor 5 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Somatostatin receptor 5 antibody . Somatostatin receptor 5 expression in wild type (WT) and Somatostatin receptor 5 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Somatostatin receptor 5 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Validation of Somatostatin receptor 5 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Somatostatin receptor 5 antibody and analyzed using BD flow cytometer.

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