This gene encodes a member of the heat shock protein 70 family, which contains both heat-inducible and constitutively expressed members. This protein belongs to the latter group, which are also referred to as heat-shock cognate proteins. It functions as a chaperone, and binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Western blotting analysis using Hsc70 antibody . Hsc70 expression in wild type (WT) and Hsc70 shRNA knockdown (KD) 293T cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Hsc70 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Flow cytometric analysis of Hsc70 expression in HT-1080 cells using Hsc70 antibody . Green, isotype control; red, Hsc70.

Immunocytochemical staining of HepG2 cells with Hsc70 antibody . Nuclei were stained blue with DAPI; Hsc70 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using Hsc70 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Hsc70 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Validation of Hsc70 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) 293T cells were stained with Hsc70 antibody and analyzed using BD flow cytometer.

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