This gene encodes one of the iron-sulfur protein (IP) components of mitochondrial NADH:ubiquinone oxidoreductase (complex I). Mutations in this gene are associated with Leigh syndrome resulting from mitochondrial complex I deficiency.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

本产品仅用作科学研究！

Validation of NDUFS3 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with NDUFS3 antibody and analyzed using CytoFLEX.

Immunocytochemical staining of HeLa cells using NDUFS3 antibody , Top panel: wild-type (WT); Bottom panal: NDUFS3 shRNA knockdown (KD). Nuclei were stained blue with DAPI; NDUFS3 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.

Flow cytometric analysis of NDUFS3 expression in HepG2 cells using NDUFS3 antibody . Green, isotype control; red, NDUFS3.

Immunocytochemical staining of HepG2 cells with NDUFS3 antibody . Nuclei were stained blue with DAPI; NDUFS3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using NDUFS3 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NDUFS3 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using NDUFS3 antibody . NDUFS3 expression in wild type (WT) and NDUFS3 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with NDUFS3 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Store at -20℃ for one year.