This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue. Multiple alternatively spliced transcript variants of this gene exist, all encoding the same protein.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of AKR1A1 expression in HepG2 cells using AKR1A1 antibody . Green, isotype control; red, AKR1A1.

Immunocytochemical staining of HepG2 cells with AKR1A1 antibody . Nuclei were stained blue with DAPI; AKR1A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Medium. Scale bar, 20 μm.

Western blotting analysis using AKR1A1 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AKR1A1 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using AKR1A1 antibody . AKR1A1 expression in wild type (WT) and AKR1A1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AKR1A1 antibody and HRP-conjugated goat anti-mouse secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

Validation of AKR1A1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with AKR1A1 antibody and analyzed using BD flow cytometer.

Immunocytochemical staining of HeLa cells using AKR1A1 antibody , Top panel: wild-type (WT); Bottom panal: AKR1A1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; AKR1A1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.

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