This gene encodes a calcium-binding mitochondrial carrier protein. The encoded protein localizes to the mitochondria and is involved in the exchange of aspartate for glutamate across the inner mitochondrial membrane. Polymorphisms in this gene may be associated with autism, and mutations in this gene may also be a cause of global cerebral hypomyelination. Alternatively spliced transcript variants have been observed for this gene.

推荐稀释比 WB: 1/1000-1/5000; FC: 1/200-1/2000; ICC/IF: 1/100-1/1000

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Flow cytometric analysis of Solute carrier family 25 member 12 expression in C2C12 cells using Solute carrier family 25 member 12 antibody . Green, isotype control; red, Solute carrier family 25 member 12.

Immunocytochemical staining of C2C12 cells with Solute carrier family 25 member 12 antibody . Nuclei were stained blue with DAPI; Solute carrier family 25 member 12 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using solute carrier family 25 member 12 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with solute carrier family 25 member 12 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using Solute carrier family 25 member 12 antibody . Solute carrier family 25 member 12 expression in wild type (WT) and Solute carrier family 25 member 12 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Solute carrier family 25 member 12 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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