LEFTY1: This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which plays a role in left-right asymmetry determination of organ systems during development. This gene is closely linked to both a related family member and a related pseudogene.

推荐稀释比 WB: 1/1000-1/5000; ICC/IF: 1/100-1/1000

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Immunocytochemical staining of HepG2 cells with LEFTY1 + LEFTY2 antibody . Nuclei were stained blue with DAPI; LEFTY1 + LEFTY2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using LEFTY1 + LEFTY2 antibody . Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with LEFTY1 + LEFTY2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using FeQ™ ECL Substrate Kit .

Western blotting analysis using LEFTY1 + LEFTY2 antibody . LEFTY1 + LEFTY2 expression in wild type (WT) and LEFTY1 + LEFTY2 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with LEFTY1 + LEFTY2 antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively. Image was developed using NaQ™ ECL Substrate Kit .

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